EDUCATION
Fermentors, Conditioning or Lagering Vessels, Brite or Serving Tanks
PROCESS OVERVIEW
Througout the entire production process, product in tanks is easily tested for the presence of spoilage bacteria and wild yeast using FastOrange bouillon broth products.
Combine finished product in a 1:1 ratio with FastOrange B (2036-1) or FastOrange Wild Yeast (2039-1) and incubate.
Contaminated product will change the media color from violet to yellow and may form sediment or haze.
ESSENTIAL
Follow ASEPTIC SAMPLING TECHNIQUE when collecting samples and create a STERILE FIELD when performing the testing steps below.
TARGET SPOILERS
LACTIC ACID BACTERIA
Lactobacillus
Pediococcus
STRICTLY ANAEROBIC BACTERIA
Megasphera
Pectinatus
WILD YEAST
Brettanomyces
Diastaticus
IMPORTANT NOTICE
DO NOT AUTOCLAVE
FASTORANGE PRODUCTS.
They are sterile and ready-to-use
directly out of their container.
FAST ORANGE USES
More Testing Topics Coming Soon…
> Chasing Brettanomyces
> Chasing Diastaticus
> Isolating Wild Yeast
> PCR Pre-Enrichment
> Propagating Brettanomyces
> Testing Barrels
> Testing CIP Rinse Water
> Testing Draft Systems
> Testing Gaskets
> Testing Hoses
> Testing Pitching Yeast
EQUIPMENT*
Needed to Collect Samples
- Spray or wash bottle of 70% isopropyl alcohol
- Sterile swabs or nylon brush
- Butane torch
- Sterile flask or Whirl-Pak with volume of > 200 ml
STEP 1
Collect Samples
-Follow the SOP for collecting aseptic samples using a sample valve.
STEP 2
Prepare Work Area
-Follow the SOP for preparing the work area for sample processing.
EQUIPMENT*
Needed to Process Samples
- Spray or wash bottle of 70% isopropyl alcohol
- Clean paper towels
- Bunsen burner or lamp
- Sample flask or Whirl-Paks
- Appropriate FastOrange media
*Consult lab equipment list
STEP 3
Prepare the Sterile Area
-Follow the SOP for preparing a sterile area.
STEP 4
Prepare the Medium
-Working inside the sterile area…
-Pipette or pour 50 ml of 2036 or 2039 media into the number of sterile flasks or whirl-paks that equal the number of tests being performed
-Close or cover media containers
-Re-seal and set FastOrange media bottles aside.
STEP 4
Combine Product and Medium
-Working inside the sterile area…
-Gently swirl the first sample container to re-suspend any sediment that may have precipitated out during storage
-Pour or pipette 50 ml of re-suspended product into the first FastOrange flask, bottle, or whirl-pak
-Close or seal containers with sample + medium mixture
NOTE: If using Whirl-Paks, push as much air as possible out of the bag before sealing the top.
-Repeat if testing the same sample across multiple FastOrange media, if running duplicates of the same medium, or if processing samples from multiple tanks
-Extinguish the Bunsen burner and clean the sample processing area.
