USING FAST ORANGE
Testing Cans & Bottles
Craft beverages packaged in cans or bottles are easily tested for the presence of spoilage bacteria and wild yeast using FastOrange bouillon broth products.
Combine finished product in a 1:1 ratio with FastOrange B (2036-1) or FastOrange Wild Yeast (2039-1) and incubate.
Contaminated product will change the media color from violet to yellow and may form sediment or haze.
Enriched samples can then be further tested via PCR.
Create a STERILE FIELD and follow ASEPTIC SAMPLING TECHNIQUE when performing the testing steps below
LACTIC ACID BACTERIA
STRICTLY ANAEROBIC BACTERIA
DO NOT AUTOCLAVE
They are sterile and ready-to-use
directly out of their container.
FAST ORANGE USES
More Testing Topics Coming Soon…
> Chasing Brettanomyces
> Chasing Diastaticus
> Isolating Wild Yeast
> PCR Pre-Enrichment
> Propagating Brettanomyces
> Testing Barrels
> Testing CIP Rinse Water
> Testing Draft Systems
> Testing Gaskets
> Testing Hoses
> Testing Pitching Yeast
Needed to Perform Test
- Spray or wash bottle of 70% isopropyl alcohol
- Clean paper towels
- Bunsen burner
- Butane torch or lighter
- Sterile flask or Whirl-Pak with volume of > 200 ml
- Appropriate FastOrange media
*Consult lab equipment list
-Working inside the area just cleaned, create a sterile field then…
–Pipette or pour 50 ml of 2036 or 2039 media into the number of sterile flasks or whirl-paks that equal the number of tests being performed.
-Close or cover media containers.
NOTE: Once opened, we recommend dispensing all of the original bottle’s contents.
-Tubes, bottles, or Whirl-Paks filled with FastOrange, when stored at room temperature and away from light, can be kept ready for use for weeks or months.
Prepare Packaged Products
-Clean and rinse outside of all cans or bottles to be tested, including lids or caps
-Spray top of first can or bottle, including lid or cap, with 70% isopropyl alcohol
-Set alcohol bottle aside, safely away from working area
-Ignite the top of the container and allow flame to extinguish on its own
-Clean hands with soap and water then apply hand disinfectant.
Combine Product and Medium
-Working inside a sterile field, carefully open the first container and discard ~20% of the volume into a bucket or sink
-Gently swirl the remaining volume to re-suspend any sediment that may have precipitated out during storage
-Pour 50 ml of re-suspended product into the first FastOrange flask or pak
-Repeat if testing the same package across multiple FastOrange media or if running duplicates of the same medium.
-Cover flask or seal whirl-pak and place upright in a warm area or incubator
-To prevent spills or leaks, place flasks or paks inside a shallow pan or bucket before heating.
-Incubate at 25 ± 2 °C (77 ± 4 °F) for 3-7 days
-Download this SOP
-PIKA Media User Manual (2036-1)
-PIKA Media User Manual (2039-1)